Citation
Regional differences in bone collagen δ13C and δ15N of Pleistocene mammoths: Implications for paleoecology of the mammoth steppe.
Szpak Paul, Darren R. Gröcke, Régis Debruyne, Ross D.E. MacPhee, R.D. Guthrie, Grant D. Zazula, W.P. Patterson, & Hendrik N. Poinar.
Palaeogeography, Palaeoclimatology, Palaeoecology 2010, 286:88-96.
doi: 10.1016/j.palaeo.2009.12.009 Read more…
Publications, article de revue internationale
analyse isotopique, Mammuthus, préservation/dégradation
Citation
Anatomy and phylogenetic value of the mandibular and coronoid canals and their associated foramina in proboscideans (Mammalia).
Ferretti, Marco & Régis Debruyne.
Zoological Journal of the Linnaean Society 2010, sous presse. Read more…
Publications, article de revue internationale
Elephantidae, Mammuthus, paléontologie
Citation
A Quantitative Approach to Detect and Overcome PCR Inhibition in Ancient DNA Extracts.
King, Christine E., Régis Debruyne, Melanie Kuch, Carsten Schwarz, & Hendrik N. Poinar.
Biotechniques 2009, 47(5):351-356.
doi 10.2144/000113244 Read more…
Publications, article de revue internationale
inhibition, Liens ADN ancien, PCR quantitative, préservation/dégradation
Citation
The “time-dependency of molecular rates of evolution revisited”: how well-calibrated paleontological evidence can clear the mess in the Bayesian debate generated by poorly calibrated uninformative molecular data.
Régis Debruyne & Hendrik Poinar.
Journal of Vertebrate Paleontology 2009, 29(Supplement to 3):86A. Read more…
Actes de congrès, Publications
Citation
Revolutions and crises in Ancient DNA: at the dawn of large-scale paleogenomics, what have we learnt from our previous mistakes?
Régis Debruyne
In Program and Abstracts of The International Meeting of the French Network of Paleogenetics “Ancient DNA : from mitochondrial to nuclear DNA, from the evolution of populations to the selection of characters”, IJM, Paris:14 Read more…
Actes de congrès, Publications
Citation
Comment on “DNA from Pre-Clovis Human Coprolites in Oregon, North America”.
Poinar Hendrik N., Stuart Fiedel, Kirsti Bos, Chrsitine E. King, Alison M. Devault, Melanie Kuch, & Régis Debruyne.
Science 2009, 325(5937):148.
DOI: 10.1126/science.1168182 Read more…
Publications, article de revue internationale
Citation
Time-Dependency of Molecular Rates in Ancient DNA Datasets, a Sampling Artifact?
Régis Debruyne & Hendrik N. Poinar
Syst Biol 2009 58(3): 348-360.
doi:10.1093/sysbio/syp028 Read more…
Publications, article de revue internationale
Citation
What controls for the new era of high-throuput sequencing in ancient DNA?
Debruyne Régis.
CAPA/ACAP Newsletter 2009(1):14. Read more…
Actes de congrès, Publications
High-throuput sequencing, méthode 454
Citation
New insights from old bones: DNA preservation and degradation in permafrost preserved mammoth remains.
Schwarz Carsten, Régis Debruyne, Melanie Kuch, Elizabth McNally, Henry Schwarcz, Andrew Aubrey, Jeffrey Bada, Hendrik N. Poinar.
Nucleic Acids Research 2009, Advance Online Access 1-15.
Abstract
Despite being plagued by heavily degraded DNA in palaeontological remains, most studies addressing the state of DNA degradation have been limited to types of damage which do not pose a hindrance to Taq polymerase during PCR. Application of serial qPCR to the two fractions obtained during extraction (demineralization and protein digest) from six permafrost mammoth bones and one partially degraded modern elephant bone has enabled further insight into the changes which endogenous DNA is subjected to during diagenesis. We show here that both fractions exhibit individual qualities in terms of the prevailing type of DNA (i.e. mitochondrial versus nuclear DNA) as well as the extent of damage, and in addition observed a highly variable ratio of mitochondrial to nuclear DNA among the six mammoth samples. While there is evidence suggesting that mitochondrial DNA is better preserved than nuclear DNA in ancient permafrost samples, we find the initial DNA concentration in the bone tissue to be as relevant for the total accessible mitochondrial DNA as the extent of DNA degradation post-mortem. We also evaluate the general applicability of indirect measures of preservation such as amino-acid racemization, bone crystallinity index and thermal age to these exceptionally well-preserved samples.
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Publications
ADN mitochondrial, ADN nucléaire, Liens ADN ancien, Mammuthus, PCR quantitative, permafrost, préservation/dégradation
Debruyne R., C. Schwarz, H.N. Poinar.
2008. Science 322(5903):857a
Read more…
Publications
Liens ADN ancien, Mammuthus
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