journal.pone.0051263.g003

Molecular Identification and Metagenomics Using Complete Mitochondrial Genomes

Researchers sorely need markers and approaches for biodiversity exploration (both specimen linked and metagenomics) using the full potential of next generation sequencing technologies (NGST). Currently, most studies rely on expensive multiple tagging, PCR primer universality and/or the use of few markers, sometimes with insufficient variability. We propose a novel approach for the isolation and sequencing of a universal, useful and popular marker across distant, non-model metazoans: the complete mitochondrial genome.

vanX

Antibiotic resistance is ancient

The discovery of antibiotics more than 70 years ago initiated a period of drug innovation and implementation in human and animal health and agriculture. These discoveries were tempered in all cases by the emergence of resistant microbes. This history has been interpreted to mean that antibiotic resistance in pathogenic bacteria is a modern phenomenon; this view is reinforced by the fact that collections of microbes that predate the antibiotic era are highly susceptible to antibiotics.

szpak

Regional Differences in Bone Collagen d13C and d15N of Pleistocene Mammoths: Implications for Palaeoecology of the Mammoth Steppe

Citation Regional differences in bone collagen δ13C and δ15N of Pleistocene mammoths: Implications for paleoecology of the mammoth steppe. Szpak Paul, Darren R. Gröcke, Régis Debruyne, Ross D.E. MacPhee, R.D. Guthrie, Grant D. Zazula, W.P. Patterson, & Hendrik N. Poinar. Palaeogeography Palaeoclimatology Palaeoecology 2010, 286(1-2):88-96. doi: 10.1016/j.palaeo.2009.12.009

New insights from old bones: DNA preservation and degradation in permafrost preserved mammoth remains

Citation New insights from old bones: DNA preservation and degradation in permafrost preserved mammoth remains. Schwarz Carsten, Régis Debruyne, Melanie Kuch, Elizabth McNally, Henry Schwarcz, Andrew Aubrey, Jeffrey Bada, Hendrik N. Poinar. Nucleic Acids Research 2009, 37(10): 3215–3229. DOI:10.1093/nar/gkp159 Abstract Despite being plagued by heavily degraded DNA in palaeontological remains, most studies addressing the state…